Jorgensen RA, Cluster PD, English J, Que QD, Napoli CA (1996) Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs antisense constructs and single-copy vs complex T-DNA sequences. Plant Mol Biol 31:957–973. CrossRef CAS PubMed Google Scholar
In their study, they attempted to discern whether the high throughput sequencing reads originated from the sense strand by second-strand synthesis (from the DNA polymerase) or the antisense strand by first-strand synthesis (from the reverse transcriptase) . Since both strands displayed a similar bias pattern, they concluded that the second The ratios of numbers of LINE copies in sense vs. antisense are at most ~2-fold, much lower than the ~5-fold ratio for “far” Can-SINEs or the ~11-fold for “near” Can-SINEs. There are an equal number of MIRs inserted in sense and antisense orientation in the “far” region of 3’UTRs. Antisense oligonucleotides (ASOs) have emerged as a promising novel drug modality that aims to address unmet medical needs. A record of six ASO drugs have been approved since 2016, and more candidates are in clinical development. ASOs are the most advanced class within the RNA- based therapeutics field. R.A. Jorgensen P.D. Cluster J. English Q. Que C.A. Napoli (1996) ArticleTitle Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences Plant Mol. Biol 31 957–973 Occurrence Handle 10.1007/BF00040715 Occurrence Handle 8843939Antisense long non-coding RNAs (antisense lncRNAs), transcribed from the opposite strand of genes with either protein coding or non-coding function, were reported recently to play a crucial role in the process of tumor onset and development. Functionally, antisense lncRNAs either promote or suppress cancer cell proliferation, migration, invasion, and chemoradiosensitivity. Mechanistically
Sense and antisense strands of DNA [9:55] In genetics, a sense strand, or coding strand, is the segment of double-stranded DNA running from 5' to 3' that is complementary to the antisense strand of DNA, which runs from 3' to 5'. The sense strand is the strand of DNA that has the same sequence as the mRNA, which takes the antisense strand as itsI am just not sure whether to use repair oligo in sense or antisense orientation with sense sgRNA. Thorben, to my knowledge it matters when you have an intact gRNA recognition sequence in your.